What is peptide Impurities?
What is peptide Impurities? By Somabiotech Co., Ltd Most of our customers often ask what other substances (impurities) are present in their peptide samples. This depends significantly on whether or not the sample was purified by reverse-phase HPLC. The following table summarizes the difference between the two cases. Impurities found in Non-Purified Peptides Deletion sequences Truncation sequences Incompletely deprotected sequences Sequences modified during cleavage (reattachment of protecting groups at other locations on the peptide) DTT (dithiothreitol) TFA (trifluoroacetic acid) Acetic acid Peptides that have undergone side reactions such as proline isomerization or isoaspartimide formation, etc. (Note that the impurities are both peptides and non-peptides.) Impurities found in Purified Peptides Deletion sequences Truncation sequences Incompletely deprotected sequences Sequences modified during cleavage (reattachment of protecting groups at other locations on t